Prep HPLC Column, RP C4, Wide Pore, 5um, 300A. 21.2mm ID x 50mm Length. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for the purification and isolation of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides enhanced accessibility for large biomolecules while maintaining the reduced hydrophobic retention characteristics of C4 chemistry. The wide pore design is particularly beneficial for preparative bioseparations where recovery, loading capacity, and chromatographic efficiency are critical.
68504-P21-50-3M is a preparative HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 21.2 mm ID x 50 mm format. The large internal diameter provides substantial sample loading capacity, while the shorter 50 mm column length is optimized for faster purification cycles and higher throughput. The 300 Å pore structure improves accessibility and mass transfer for proteins, peptides, enzymes, and other large biomolecules. Typical operating flow rates are approximately 15–30 mL/min depending on analyte characteristics, mobile phase composition, and system capabilities.
This column is particularly useful for the purification of proteins, peptides, enzymes, and other large biomolecules in pharmaceutical, biopharmaceutical, and research laboratories. Compared to more hydrophobic C8 and C18 phases, the C4 bonded phase generally provides lower retention, which can improve recovery and purification efficiency for larger analytes. The 21.2 mm ID format is ideal for preparative applications requiring high sample loading capacity and efficient fraction collection. A specialized preparative HPLC system is required for operation.
Key Benefits
RP C4™ Wide Pore stationary phase
USP L26 equivalent chemistry
Butyl (C4) bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
High sample loading capacity
Optimized for proteins and peptides
Lower hydrophobic retention than C8 phases
Suitable for preparative bioseparations
Typical Applications
Preparative HPLC
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Fraction collection
Biopharmaceutical purification
Process development
Method development
Laboratory-scale production
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C4™ Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 21.2 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 15–30 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve accessibility for larger analytes
Provides less retention than C8 and C18 stationary phases
High loading capacity for preparative-scale purification
The 21.2 mm ID format requires a specialized preparative HPLC system
Typical operating flow rates are approximately 15–30 mL/min
5 µm particles provide lower operating backpressure and robust performance
Fully double end capped to minimize secondary interactions
The 50 mm length is ideal for fast purification cycles and high-throughput preparative work
Excellent choice for preparative protein and peptide purification
This is a Special Order Item